igg2c fitc antibody Search Results


fluorescein isothiocyanate fitc conjugated goat anti rabbit antibody  (ProSci Incorporated)
90
ProSci Incorporated fluorescein isothiocyanate fitc conjugated goat anti rabbit antibody
Fluorescein Isothiocyanate Fitc Conjugated Goat Anti Rabbit Antibody, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescein isothiocyanate fitc conjugated goat anti rabbit antibody/product/ProSci Incorporated
Average 90 stars, based on 1 article reviews
fluorescein isothiocyanate fitc conjugated goat anti rabbit antibody - by Bioz Stars, 2026-02
90/100 stars
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fitc-conjugated rat igg2c  (MBL International)
90
MBL International fitc-conjugated rat igg2c
Development of an M cell–targeted mucosal vaccine with NKM 16–2-4. <t>(A)</t> <t>FITC-conjugated</t> NKM 16–2-4, but not FITC-conjugated control rat IgG, was specifically attached to the apical surfaces of M cells in FAE of PPs within 10 min of inoculation in an intestinal loop assay. The NKM 16–2-4 was subsequently taken up into the cytoplasmic regions of M cells within 30 min and reached the basal membrane of M cells within 4 h. Bars, 10 μm. (B) TT conjugated with NKM 16–2-4 effectively induced high-level, TT-specific serum IgG and fecal IgA responses, unlike TT conjugated with control rat IgG or UEA-1. Furthermore, the levels were superior to those in mice immunized with 10 times the amount of noncoupled TT (500 μg). *, P < 0.01, Tukey's t test. (C) BT-conjugated NKM 16–2-4, but not BT-conjugated control rat IgG, induced brisk botulinum toxin–specific serum IgG and fecal IgA responses. (D) Mice orally immunized with BT-conjugated NKM 16–2-4 were protected from an i.p. challenge with 10,000× LD 50 type A botulinum toxin. Data are expressed as the mean ± the SD.
Fitc Conjugated Rat Igg2c, supplied by MBL International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fitc-conjugated rat igg2c/product/MBL International
Average 90 stars, based on 1 article reviews
fitc-conjugated rat igg2c - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


Development of an M cell–targeted mucosal vaccine with NKM 16–2-4. (A) FITC-conjugated NKM 16–2-4, but not FITC-conjugated control rat IgG, was specifically attached to the apical surfaces of M cells in FAE of PPs within 10 min of inoculation in an intestinal loop assay. The NKM 16–2-4 was subsequently taken up into the cytoplasmic regions of M cells within 30 min and reached the basal membrane of M cells within 4 h. Bars, 10 μm. (B) TT conjugated with NKM 16–2-4 effectively induced high-level, TT-specific serum IgG and fecal IgA responses, unlike TT conjugated with control rat IgG or UEA-1. Furthermore, the levels were superior to those in mice immunized with 10 times the amount of noncoupled TT (500 μg). *, P < 0.01, Tukey's t test. (C) BT-conjugated NKM 16–2-4, but not BT-conjugated control rat IgG, induced brisk botulinum toxin–specific serum IgG and fecal IgA responses. (D) Mice orally immunized with BT-conjugated NKM 16–2-4 were protected from an i.p. challenge with 10,000× LD 50 type A botulinum toxin. Data are expressed as the mean ± the SD.

Journal: The Journal of Experimental Medicine

Article Title: A novel M cell–specific carbohydrate-targeted mucosal vaccine effectively induces antigen-specific immune responses

doi: 10.1084/jem.20070607

Figure Lengend Snippet: Development of an M cell–targeted mucosal vaccine with NKM 16–2-4. (A) FITC-conjugated NKM 16–2-4, but not FITC-conjugated control rat IgG, was specifically attached to the apical surfaces of M cells in FAE of PPs within 10 min of inoculation in an intestinal loop assay. The NKM 16–2-4 was subsequently taken up into the cytoplasmic regions of M cells within 30 min and reached the basal membrane of M cells within 4 h. Bars, 10 μm. (B) TT conjugated with NKM 16–2-4 effectively induced high-level, TT-specific serum IgG and fecal IgA responses, unlike TT conjugated with control rat IgG or UEA-1. Furthermore, the levels were superior to those in mice immunized with 10 times the amount of noncoupled TT (500 μg). *, P < 0.01, Tukey's t test. (C) BT-conjugated NKM 16–2-4, but not BT-conjugated control rat IgG, induced brisk botulinum toxin–specific serum IgG and fecal IgA responses. (D) Mice orally immunized with BT-conjugated NKM 16–2-4 were protected from an i.p. challenge with 10,000× LD 50 type A botulinum toxin. Data are expressed as the mean ± the SD.

Article Snippet: In brief, after a blocking step with 1% BSA, 7-μm fixed frozen sections or fixed tissues containing PPs were stained with 5 μg/ml FITC-conjugated NKM 16–2-4 or FITC-conjugated isotype control (FITC-conjugated rat IgG2c; MBL International) and 1 μg/ml tetrarhodamine isothiocyanate–conjugated UEA-1 (Vector Laboratories).

Techniques:

Effective uptake and universality of the M cell–targeted mucosal vaccine. (A) Immunocytochemical analysis showed that an M cell–targeted OVA vaccine composed of Alexa Fluor 647–conjugated OVA, FITC-conjugated avidin, and NKM 16–2-4 specifically reacted with isolated UEA-1–positive M cells. (B) In an intestinal loop assay, the M cell-targeted OVA specifically attached to the apical surfaces of M cells (red arrows) and was immediately taken up into the cytoplasmic regions of M cells. Bars, 10 μm. (C) Orally administered OVA-conjugated NKM 16–2-4 effectively induced an OVA-specific serum IgG response, whereas an OVA-conjugated control rat IgG did not. Data are expressed as the mean ± the SD.

Journal: The Journal of Experimental Medicine

Article Title: A novel M cell–specific carbohydrate-targeted mucosal vaccine effectively induces antigen-specific immune responses

doi: 10.1084/jem.20070607

Figure Lengend Snippet: Effective uptake and universality of the M cell–targeted mucosal vaccine. (A) Immunocytochemical analysis showed that an M cell–targeted OVA vaccine composed of Alexa Fluor 647–conjugated OVA, FITC-conjugated avidin, and NKM 16–2-4 specifically reacted with isolated UEA-1–positive M cells. (B) In an intestinal loop assay, the M cell-targeted OVA specifically attached to the apical surfaces of M cells (red arrows) and was immediately taken up into the cytoplasmic regions of M cells. Bars, 10 μm. (C) Orally administered OVA-conjugated NKM 16–2-4 effectively induced an OVA-specific serum IgG response, whereas an OVA-conjugated control rat IgG did not. Data are expressed as the mean ± the SD.

Article Snippet: In brief, after a blocking step with 1% BSA, 7-μm fixed frozen sections or fixed tissues containing PPs were stained with 5 μg/ml FITC-conjugated NKM 16–2-4 or FITC-conjugated isotype control (FITC-conjugated rat IgG2c; MBL International) and 1 μg/ml tetrarhodamine isothiocyanate–conjugated UEA-1 (Vector Laboratories).

Techniques: Avidin-Biotin Assay, Isolation